National Academy of Sciences, Proceedings of the National Academy of Sciences, 51(113), 2016
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Significance Genome-scale models of metabolism are important tools for metabolic engineering and production strain development. We present an experimentally validated and manually curated model of metabolism in Synechococcus elongatus PCC 7942 that ( i ) leads to discovery of unique metabolic characteristics, such as the importance of a truncated, linear TCA pathway, ( ii ) highlights poorly understood areas of metabolism as exemplified by knowledge gaps in nucleotide salvage, and ( iii ) accurately quantifies light input and self-shading. We now have a metabolic model that can be used as a basis for metabolic design in S. elongatus .