Published in

National Academy of Sciences, Proceedings of the National Academy of Sciences, 43(113), p. 12076-12081, 2016

DOI: 10.1073/pnas.1605086113

Links

Tools

Export citation

Search in Google Scholar

Controlling uncertainty in aptamer selection

Journal article published in 2016 by Fabian Spill, Zohar B. Weinstein ORCID, Atena Irani Shemirani, Nga Ho, Darash Desai, Muhammad H. Zaman
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

Full text: Download

Red circle
Preprint: archiving forbidden
Green circle
Postprint: archiving allowed
Upload
Red circle
Published version: archiving forbidden
Policy details
Data provided by SHERPA/RoMEO

Abstract

Significance Oligonucleotide aptamers have increasing applications as a class of molecules that bind with high affinity and specificity to a target. Aptamers are typically selected from a large pool of random candidate nucleic acid libraries through competition for the target. Using a stochastic hybrid model, we are able to study the combined impact of important evolutionary success factors such as competition, randomness, and changes in the environment. Whereas the environment may be tuned with experimental parameters such as target concentration, competition varies with differences in the initial distribution of aptamer–target binding affinities, and random events can eliminate even the ligands with the highest affinity.