Published in

Nature Research, Nature Communications, 1(5), 2014

DOI: 10.1038/ncomms4309

Links

Tools

Export citation

Search in Google Scholar

Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography

Journal article published in 2014 by Uwe Weierstall, Daniel James, Chong Wang, Thomas A. White ORCID, Dingjie Wang, Wei Liu, R. Bruce Doak, R. Bruce Doak, John Ch H. Spence, Garrett Nelson, Petra Fromme, Raimund Fromme, Ingo Grotjohann, Christopher Kupitz, Nadia A. Zatsepin and other authors.
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

Full text: Download

Green circle
Preprint: archiving allowed
Upload
Red circle
Postprint: archiving forbidden
Green circle
Published version: archiving allowed
Upload
Policy details
Data provided by SHERPA/RoMEO

Abstract

Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously-renewed source of material for serial femtosecond crystallography. Data collected from sub-10 μm-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.