XTEND: Extending the depth of field in cryo soft X-ray tomography
Journal article published in 2017 by
Joaquín Otón 
,
Eva Pereiro,
José J. Conesa ,
Francisco J. Chichón,
Daniel Luque,
Javier M. Rodríguez,
Ana J. Pérez-Berná,
Carlos Oscar S. Sorzano ,
Joanna Klukowska,
Gabor T. Herman,
Javier Vargas,
Roberto Marabini,
José L. Carrascosa,
José M. Carazo
,
Eva Pereiro,
José J. Conesa ,
Francisco J. Chichón,
Daniel Luque,
Javier M. Rodríguez,
Ana J. Pérez-Berná,
Carlos Oscar S. Sorzano ,
Joanna Klukowska,
Gabor T. Herman,
Javier Vargas,
Roberto Marabini,
José L. Carrascosa,
José M. Carazo
This paper is made freely available by the publisher.
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Abstract
AbstractWe have developed a new data collection method and processing framework in full field cryo soft X-ray tomography to computationally extend the depth of field (DOF) of a Fresnel zone plate lens. Structural features of 3D-reconstructed eukaryotic cells that are affected by DOF artifacts in standard reconstruction are now recovered. This approach, based on focal series projections, is easily applicable with closed expressions to select specific data acquisition parameters.