Trichoderma harzianum T6776 is a promising beneficial isolate whose effects consist of growth promotion, positive response of photosynthetic activity, hormonal signaling, and carbon partitioning in tomato, coupled with biocontrol of plant pathogens. Here, we present the first genome assembly of T6776, providing a useful platform for the scientific community. FOOTNOTES Address correspondence to Sabrina Sarrocco, sabrina.sarrocco{at}unipi.it. ↵* Present address: Riccardo Baroncelli, Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, Université de Bretagne Occidentale, Brest, France. This article is dedicated to Maurizio Forti, friend and devoted technician at the University of Pisa, who passed away in December 2013. Citation Baroncelli R, Piaggeschi G, Fiorini L, Bertolini E, Zapparata A, Pè ME, Sarrocco S, Vannacci G. 2015. Draft whole-genome sequence of the biocontrol agent Trichoderma harzianum T6776. Genome Announc 3(3):e00647-15. doi:10.1128/genomeA.00647-15. Received 14 May 2015. Accepted 18 May 2015. Published 11 June 2015. Copyright © 2015 Baroncelli et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license.