The chemical constituents of Ligularia wilsoniana were studied for the first time, using four samples collected in Chongqing City, China. Two new compounds, 6β-(2′,3′-epoxy-2′-methylpropanoyloxy)-1β,10β-epoxyfuranoeremophilane and 11αH-6β-(2′-hydroxymethylacryloyloxy)-1β,10β;7β,8β-diepoxyeremophilanolide, as well as eight known compounds, were isolated from one of the samples, while three to four known furanoeremophilanes were isolated from the other three samples. No compound was common to the two classes of the samples, demonstrating the presence of at least two chemotypes of this species.