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MOESM5 of Transcriptional reprogramming in yeast using dCas9 and combinatorial gRNA strategies

This paper is available in a repository.
This paper is available in a repository.

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Abstract

Additional file 5: Figure S4. Transcriptional regulation vs. nucleosome occupancy. Relative MFI (deviation from ’no gRNA’ control) is shown on second axis vs. predicted nucleosome occupancy on first axis for 12 yeast promoters. Predicted nucleosome occupancies were based on Kaplan et al. [33] and are the averaged values between the 5’- and 3’-most occupancy values of gRNAs. Low predicted nucleosome occupancy scores relate to lower nucleosome densities at a given position. Dark grey symbolizes regulation with MCP-VPR (activation) and light grey with PCP-Mxi1 (repression). Results are based on at least three biological replicates.