Additional file 3: Figure S2. Time-dependent regulation of reporter gene expression. Data were obtained with a BioLector from the same cultures as used in Supplementary Fig. S1. Indicated strains were targeted at pHMG1 or pOLE1 as in Fig. 1C with controls (ctrl) expressing dCas9 and no gRNA. Data were collected for ~47 hrs and are presented as the average of three biological replicates. A. MFI from strains targeted with the constitutive system is presented per DCW/L (dry cell weight per liter) as a function of time. Blue; control. Green; activation (MCP-VPR). Red; repression (PCP-Mxi1). B. MFI from cultures added 250 ng/mL aTc to activate the inducible system is presented per OD (OD600) over time. Blue; control. Green; activation (dCas9-VPR). Red; repression (dCas9-Mxi1).