Additional file 2: Figure S1. Growth kinetics during transcriptional regulation of pHMG1 and pOLE1. Indicated strains were the same as in Fig. 1C targeted for regulation at pHMG1 or pOLE1, with controls (ctrl) expressing dCas9 and no gRNA. Growth was monitored over ~47 hours with a BioLector, and presented data is the average of three biological replicates. A. Strains that harbored the constitutive system were monitored for increasing dry cell weight (DCW) per liter (g/L) for controls (blue), activation with MCP-VPR (green), and repression with PCP-Mxi1 (red). B. Strains that contained the inducible system were cultured with 250 ng/mL aTc, and OD600 was monitored. Blue indicates control cultures (dCas9), green activation (dCas9-VPR), and red repression (dCas9-Mxi1).