Percentages of CD4+ and CD8+ CAR T cells without a hinge domain both tended to be stable throughout the in vitro culture period. Flow cytometric analysis of the percentage of CD4+ and CD8+ (A) 19.28z T, (B) Meso.28z T, (C) PSCA.28z T, (D) HER2.28z T, and (E) GFP control T cells during the in vitro culture period. The data are representative of independent experiments verified with cells from over three individual healthy human donors. (JPG 924Â kb)