The contribution of natural sources in particulate matter (PM) concentrations has been assessed for 5 Southern European cities: Porto (Portugal), Barcelona (Spain), Milan and Florence (Italy) and Athens (Greece). A database on the impact of natural source has been compiled, including concentrations of PM and chemical tracers used for the identification and quantification of African dust and sea salt contributions, as well as the calculated African net dust, and sea salt concentrations for each city. In addition, wildfires’ contribution is provided for Porto. Both PM 10 and PM 2.5 concentrations are reported for a total of six sites: Porto urban traffic site, POR-TR Barcelona urban background site, BCN-UB Milano urban background site, MLN-UB Florence urban background site, FI-UB Athens suburban site, ATH-SUB Athens urban traffic site, ATH-TR.