AbstractCholesterol is a key component of cell membranes with a proven modulatory role on the function and ligand-binding properties of G-protein-coupled receptors (GPCRs). Crystal structures of prototypical GPCRs such as the adenosine A_2A receptor (A_2AR) have confirmed that cholesterol finds stable binding sites at the receptor surface suggesting an allosteric role of this lipid. Here we combine experimental and computational approaches to show that cholesterol can spontaneously enter the A_2AR-binding pocket from the membrane milieu using the same portal gate previously suggested for opsin ligands. We confirm the presence of cholesterol inside the receptor by chemical modification of the A_2AR interior in a biotinylation assay. Overall, we show that cholesterol’s impact on A_2AR-binding affinity goes beyond pure allosteric modulation and unveils a new interaction mode between cholesterol and the A_2AR that could potentially apply to other GPCRs.