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We demonstrate the supramolecular self-assembly of {porphyrin-5,10,15,20-tetrayltetrakis[(benzene-5,1,3-triyl)bis (methylene)]}octakis(phosphonic acid) 1 in the presence of melamine and spermine. The morphology of 1 in the presence of melamine and spermine was different, indicated that change in analytes could be used to control nanostructures. UV-vis and fluorescence spectroscopic methods were employed to investigate aggregation mode. The self-assembled nanostructure was confirmed by SEM imaging and XRD measurements.