Additional file 1: Figure S1. of A novel modified-indirect ELISA based on spherical body protein 4 for detecting antibody during acute and long-term infections with diverse Babesia bovis strains
Published in 2017 by
Chungwon Chung,
Carlos Suarez,
Carey Bandaranayaka-Mudiyanselage,
Chandima-Bandara Bandaranayaka-Mudiyanselage,
Joanna Rzepka,
Heiniger Tj,
Grace Chung,
Stephen Lee,
Ethan Adams,
Grace Yun,
Susan Waldron
Abstract
Cattle sera positive by the RAP-1-based cELISA but negative by the SBP4-based MI-ELISA and IFA had negative results by Western blot analysis, suggesting possible false positive results in the cELISA. A. Molecular weight marker (48 to 180 Kd), B. K42-#21, C. W31-#Y-3, D. W31-#Y-11, E. W31-#0-3, F. W31-#Y-9, G. W31-#0-9, H. W31-#Y-10, I. W31-#Y-15, J. P21-#224, K. positive control serum with a band at 75kd representing B. bovis RAP-1 protein, J. negative control serum. Figure S2. Technical difference between the modified indirect ELISA and conventional indirect ELISA using rGST-SBP4 was illustrated in this figure. (DOCX 645 kb)