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American Society for Microbiology, Molecular and Cellular Biology, 5(21), p. 1573-1580, 2001

DOI: 10.1128/mcb.21.5.1573-1580.2001

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VAMP3 Null Mice Display Normal Constitutive, Insulin- and Exercise-Regulated Vesicle Trafficking

Journal article published in 2001 by Chunmei Yang, Silvia Mora, Jeffrey W. Ryder, Kenneth J. Coker, Polly Hansen, Allen La, Lee-Ann Allen ORCID, Jeffrey E. Pessin
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

To investigate the physiological function of the VAMP3 vesicle SNARE (v-SNARE) isoform in the regulation of GLUT4 vesicle trafficking, we generated homozygotic VAMP3 null mice by targeted gene disruption. The VAMP3 null mice had typical growth rate and weight gain, with normal maintenance of fasting serum glucose and insulin levels. Analysis of glucose disposal and insulin sensitivity demonstrated normal insulin and glucose tolerance, with no evidence for insulin resistance. Insulin stimulation of glucose uptake in isolated primary adipocytes was essentially the same for the wild-type and VAMP3 null mice. Similarly, insulin-, hypoxia-, and exercise-stimulated glucose uptake in isolated skeletal muscle did not differ significantly. In addition, other general membrane trafficking events including phagocytosis, pinocytosis, and transferrin receptor recycling were also found to be unaffected in the VAMP3 null mice. Taken together, these data demonstrate that VAMP3 function is not necessary for either regulated GLUT4 translocation or general constitutive membrane recycling.