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American Society for Microbiology, Applied and Environmental Microbiology, 23(75), p. 7542-7547, 2009

DOI: 10.1128/aem.00814-09

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Development and Application of Quantitative-PCR Tools for Subgroups of the Roseobacter Clade

Journal article published in 2009 by Alison Buchan, Mary Hadden, Marcelino T. Suzuki ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT Specific SYBR green-based quantitative-PCR assays targeting conserved regions in the 16S-23S rRNA internal transcribed spacer regions were developed for five subgroups of the environmentally abundant and biogeochemically active Roseobacter clade of marine bacteria. The assays were applied to field samples demonstrating their utility in investigations of abundant Roseobacter group phylotypes in the environment.