Accurate estimation of yield gaps is only possible for locations where high quality local data are available, which are, however, lacking in many regions of the world. The challenge is how yield gap estimates based on location-specific input data can be used to obtain yield gap estimates for larger spatial areas. Hence, insight about the minimum number of locations required to achieve robust estimates of yield gaps at larger spatial scales is essential because data collection at a large number of locations is expensive and time consuming. In this paper we describe an approach that consists of a climate zonation scheme supplemented by agronomical and locally relevant weather, soil and cropping system data. Two elements of this methodology are evaluated here: the effects on simulated national crop yield potentials attributable to missing and/or poor quality data and the error that might be introduced in scaled up yield gap estimates due to the selected climate zonation scheme. Variation in simulated yield potentials among weather stations located within the same climate zone, represented by the coefficient of variation, served as a measure of the performance of the climate zonation scheme for upscaling of yield potentials.