Massage therapies are widely employed for improving and recovering tissue functions and physical activities. It is generally believed that such therapies would promote health and well-being by many possible mechanisms, including fastening muscle blood flow, parasympathetic activity, releasing relaxation hormones and inhibiting muscle tension, neuromuscular excitability and stress hormones. Nonetheless, most of current research is based on statistics and thus qualitative, preventing the in-depth study of the effectiveness. This is partially due to the lack of appropriate tools for quantitative loading and in situ assessment of tissue performance. To address this, we develop a biomechanical device to mimic massage therapies by applying controllable mechanical forces to animal tissues during cyclic mechanical motions. The device can apply compressive loads normal to the tissue surface and generate lengthwise motion along the tissue surface. Mechanical forces are applied with controllable magnitudes, frequencies and durations. Tissue mechanical response is recorded and correlated to the loading parameters. The changes of bulk tissue compliance and viscoelastic properties under various loading conditions are evaluated. The improvement of tissue functions and inhibition of muscle inflammation are examined. The results show that the peak torque production increased after massage, which suggests the recovery of muscle functions. A reduced number of infiltrating leukocytes is also observed in the subject muscle fibers after massage. Findings of this study suggest that the biomechanical device offers a quantitative analysis of massage actions, which will help to determine the optimal range of loading conditions required for safe and effective use of massage therapies.