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Nature Research, Nature Methods, 5(9), p. 477-479, 2012

DOI: 10.1038/nmeth.1945

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Direct visualization of specifically modified extracellular glycans in living animals

Journal article published in 2012 by Matthew Attreed, Muriel Desbois, Toin H. van Kuppevelt, T. van Kuppevelt, Hannes E. Bülow
This paper is available in a repository.
This paper is available in a repository.

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Abstract

Modification patterns of the extracellular glycan heparan sulfate coordinate protein function in metazoans, yet in vivo imaging of such non-genetically encoded structures has been impossible. Here we report a transgenic method in Caenorhabditis elegans that allows direct live imaging of specific heparan sulfate modification patterns. This experimental approach reveals a dynamic and cell-specific heparan sulfate landscape and could in principle be adapted to visualize and analyze any extracellular molecule in vivo.