This work was carried out to determine the species of Calliphoridae occurring in the Tinguá Biological Reserve, Nova Iguaçu, RJ, Brazil. Four plastic traps were used to collect the flies. The traps were suspended 1.20 m above the ground for a period of 48 hours, every two weeks. Fish (sardine) in decomposition was used as bait. A total of 2,519 Calliphoridae flies were captured, belonging to 11 species: Chloroprocta idioidea (Robineau-Desvoidy), Chrysomya albiceps (Wiedemann), Chrysomya megacephala (Fabricius), Chrysomya putoria (Wiedemann), Cochliomyia macellaria (Fabricius), Hemilucilia hermanlenti (Mello), Hemilucilia segmentaria (Fabricius), Hemilucilia semidiaphana (Rondani), Lucilia eximia (Wiedemann), Paraluciliafulvinota (Bigot) and Paralucilia nigrofacialis (Mello).