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MyJove Corporation, Journal of Visualized Experiments, 5, 2007

DOI: 10.3791/230-v

MyJove Corporation, Journal of Visualized Experiments, 5

DOI: 10.3791/230

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Protocol for RNAi Assays in Adult Mosquitoes (A. gambiae)

Journal article published in 2007 by Lindsey Garver ORCID, George Dimopoulos
This paper is available in a repository.
This paper is available in a repository.

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Abstract

Reverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the Dimopoulos lab to inject dsRNA into Anopheles gambiae mosquitoes, which harbor the malaria parasite. The technique manipulating the injection setup and injecting dsRNA into the thorax is illustrated.