Published in

MyJove Corporation, Journal of Visualized Experiments, 5, 2007

DOI: 10.3791/222-v

MyJove Corporation, Journal of Visualized Experiments, 5

DOI: 10.3791/222

Links

Tools

Export citation

Search in Google Scholar

Protocol for Plasmodium falciparum Infections in Mosquitoes and Infection Phenotype Determination

Journal article published in 2007 by Zhiyong Xi, Suchismita Das, Lindsey Garver ORCID, George Dimopoulos
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

Full text: Download

Red circle
Preprint: archiving forbidden
Orange circle
Postprint: archiving restricted
Upload
Orange circle
Published version: archiving restricted
Upload
Policy details
Data provided by SHERPA/RoMEO

Abstract

Once a gene is identified as potentially refractory for malaria, it must be evaluated for its role in preventing Plasmodium infections within the mosquito. This protocol illustrates how the extent of plasmodium infections of mosquitoes can be assayed. The techniques for preparing the gametocyte culture, membrane feeding mosquitoes human blood, and assaying viral titers in the mosquito midgut are demonstrated.