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MyJove Corporation, Journal of Visualized Experiments, 5, 2007

DOI: 10.3791/221-v

MyJove Corporation, Journal of Visualized Experiments, 5

DOI: 10.3791/221

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Protocol for Mosquito Rearing (A. gambiae)

Journal article published in 2007 by Suchismita Das, Lindsey Garver ORCID, George Dimopoulos
This paper is available in a repository.
This paper is available in a repository.

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Abstract

This protocol describes mosquito rearing in the insectary. The insectary rooms are maintained at 28 degrees C and approximately 80% humidity, with a 12 hr. day/night cycle. For this procedure, you'll need mosquito cages, 10% sterile sucrose solution, paper towels, beaker, whatman filter paper, glass feeders, human blood and serum, water bath, parafilm, distilled water, clean plastic trays, mosquito food (described below), mosquito net to cover the trays, vacuum, and a collection chamber to collect adults.