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American Chemical Society, Journal of Organic Chemistry, 21(79), p. 9914-9921, 2014

DOI: 10.1021/jo5020799

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Synthesis of Base-Modified 2′-Deoxyribonucleoside Triphosphates and Their Use in Enzymatic Synthesis of Modified DNA for Applications in Bioanalysis and Chemical Biology

Journal article published in 2014 by Michal Hocek ORCID
This paper is available in a repository.
This paper is available in a repository.

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Abstract

The synthesis of 2'-deoxyribonucleoside triphosphates (dNTPs) either by classical triphosphorylation of nucleosides or by aqueous cross-coupling reactions of halogenated dNTPs is discussed. Different enzymatic methods for synthesis of modified oligonucleotides and DNA by polymerase incorporation of modified nucleotides are summarized and the applications in redox or fluorescent labelling, as well as in bioconjugations and modulation of interactions of DNA with proteins are outlined.