Signal amplification by reversible exchange (SABRE) of a substrate and parahydrogen at a catalytic center promises to overcome the inherent insensitivity of magnetic resonance. In order to apply the new approach to biomedical applications, there is a need to develop experimental equipment, in situ quantification methods, and a biocompatible solvent. We present results detailing a low-field SABRE polarizer which provides well-controlled experimental conditions, defined spins manipulations, and which allows in situ detection of thermally polarized and hyperpolarized samples. We introduce a method for absolute quantification of hyperpolarization yield in situ by means of a thermally polarized reference. A maximum signal-to-noise ratio of similar to 10(3) for 148 mu mol of substance, a signal enhancement of 10(6) with respect to polarization transfer field of SABRE, or an absolute H-1-polarization level of approximate to 10(-2) is achieved. In an important step toward biomedical application, we demonstrate H-1 in situ NMR as well as H-1 and C-13 high-field MRI using hyperpolarized pyridine (d(3)) and C-13 nicotinamide in pure and 11% ethanol in aqueous solution. Further increase of hyperpolarization yield, implications of in situ detection, and in vivo application are discussed. ; Aa3ti Times Cited:10 Cited References Count:45