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American Chemical Society, Journal of the American Chemical Society, 7(129), p. 1854-1855, 2007

DOI: 10.1021/ja067339r

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Indirect Electrochemical Sensing of DNA Hybridization Based on the Catalytic Oxidation of Cobalt (II)

Journal article published in 2007 by Di Xue, C. Michael Elliott, C. Michael Elliott, Ping Gong, David W. Grainger, Carlo A. Bignozzi ORCID, Stefano Caramori
This paper is available in a repository.
This paper is available in a repository.

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Abstract

A new electrochemical approach for detecting target DNA is described. The scheme involves the catalytic oxidation of a cobalt bipyridine “reporter molecule” in solution. Probe/target DNA duplexes (ds-DNA), bound on an ITO electrode, selectively recruit redox catalyst molecules from solution. This surface-bound catalyst “turns on” the redox chemistry of the reporter molecule which is otherwise kinetically inert to oxidation on ITO. The mode of selective catalyst binding is intercalation between base pairs of ds-DNA. With this approach, we demonstrate detection of a 20-mer DNA target oligonucleotide at picomolar concentrations with outstanding signal-to-noise.