Verocytotoxic Escherichia coli strains are responsible for Swine Oedema Disease, a serious enterotoxaemia, that causes important economic losses in pig industry. The production of a vaccine for oral administration in transgenic seeds could be a practical and efficient system to stimulate local immunity. The aim of this study was to transform tobacco plants, as a model system, for the seed-specific expression of antigenic proteins from a porcine verocytotoxic Escherichia coli strain. In addition the aim of this study was to evaluate parameters related to an immunological response and the possible adverse effects upon the oral administration of obtained tobacco seeds in mouse model. Tobacco was transformed via Agrobacteium tumefaciens with chimeric constructs containing structural parts of the major subunit FedA of the F18 adhesive fimbriae and VT2e B-subunit genes under the control of a seed specific GLOB promoter. We showed that the foreign Vt2e-B and F18 fimbriae genes are stably accumulated into storage tissue by immunostaining method. Secondly Balb-C mice, receiving transgenic tobacco seeds via oral route, showed a significant increase in IgA-positive plasma cell presence in the tunica propria compared to the control group and any adverse effects were observed. Our findings encourage future studies focusing on swine specie in order to evaluate the protective effect of transformed tobacco seeds against Escherichia coli infection.