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Nature Research, Nature Methods, 6(5), p. 535-538, 2008

DOI: 10.1038/nmeth.1210

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Next generation high density self assembling functional protein arrays

Journal article published in 2008 by Niroshan Ramachandran, Jacob V. Raphael, Eugenie Hainsworth, Gokhan Demirkan, Manuel G. Fuentes ORCID, Andreas Rolfs, Yanhui Hu, Joshua LaBaer
This paper is available in a repository.
This paper is available in a repository.

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Abstract

We report a high-density self assembling protein microarray that displays thousands of proteins, produced and captured in situ from immobilized cDNA templates. Over 1500 unique cDNAs were tested with > 90% success with nearly all proteins displaying yields within 2 fold of the mean, minimal sample variation and good day to day reproducibility. The displayed proteins revealed selective protein interactions. This method will enable various experimental approaches to study protein function in high throughput.