Published in

American Association for the Advancement of Science, Science, 6149(341), p. 1009-1012, 2013

DOI: 10.1126/science.1240985

Links

Tools

Export citation

Search in Google Scholar

Molecular Basis of Tubulin Transport Within the Cilium by IFT74 and IFT81

Journal article published in 2013 by Sagar Bhogaraju, Lukas Cajanek, Cécile Fort, Thierry Blisnick, Kristina Weber, Michael Taschner, Naoko Mizuno, Stefan Lamla, Philippe Bastin, Erich A. Nigg ORCID, Esben Lorentzen
This paper is available in a repository.
This paper is available in a repository.

Full text: Download

Green circle
Preprint: archiving allowed
Upload
Green circle
Postprint: archiving allowed
Upload
Red circle
Published version: archiving forbidden
Policy details
Data provided by SHERPA/RoMEO

Abstract

Intraflagellar transport (IFT) of ciliary precursors such as tubulin from the cytoplasm to the ciliary tip is involved in the construction of the cilium, a hairlike organelle found on most eukaryotic cells. However, the molecular mechanisms of IFT are poorly understood. Here, we found that the two core IFT proteins IFT74 and IFT81 form a tubulin-binding module and mapped the interaction to a calponin homology domain of IFT81 and a highly basic domain in IFT74. Knockdown of IFT81 and rescue experiments with point mutants showed that tubulin binding by IFT81 was required for ciliogenesis in human cells.