Dengue is endemic in more than 100 countries and is transmitted by the mosquito Aedes aegypti. The use of the symbiotic bacterium Wolbachia has become a potential biocontrol approach against dengue virus for two reasons. First, Wolbachia spreads rapidly through populations by manipulating host reproduction to its advantage. Second, Wolbachia limits viral replication in the mosquito by competing with the virus for essential host resources. Following field release in Cairns, Australia in 2011, the wMel strain of Wolbachia has successfully invaded wild mosquito populations, infecting nearly all individuals. To test whether limited dengue replication in wMel mosquitoes translates to a reduction in dengue transmission potential, we used a non-destructive assay to repeatedly quantify dengue virus in mosquito saliva. We found that wMel significantly delayed the time it took for mosquito saliva to become infectious, reduced the frequency of dengue virus that was expectorated by mosquitoes and lowered the virus titer in mosquito saliva. We also showed that wMel infection suppresses saliva production in mosquitoes that may, in part, explain our findings. The saliva-based nature of the work provides a more accurate assessment of Wolbachia’s ability to limit disease transmission and suggests that Wolbachia may have positive impacts on transmission not only by reducing the number of infectious mosquitoes in a population but also delaying the arrival of virus in the saliva.