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American Society for Microbiology, Journal of Bacteriology, 6(172), p. 3509-3511, 1990

DOI: 10.1128/jb.172.6.3509-3511.1990

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Transduction of a plasmid containing the bacteriophage D3 cos site in Pseudomonas aeruginosa.

Journal article published in 1990 by R. Sharp, E. Gertman, M. A. Farinha, A. M. Kropinski ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Plasmids harboring the cos sequences of bacteriophage D3 can be transferred, by bacteriophage D3, into Pseudomonas aeruginosa by a mechanism which is insensitive to DNase. Transducing activity was separated from the plaque-forming particles by CsCl equilibrium gradient centrifugation. Restriction endonuclease digestion patterns suggest that the transducing particles contain plasmid concatemers.