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American Society for Microbiology, Infection and Immunity, 5(69), p. 3438-3441, 2001

DOI: 10.1128/iai.69.5.3438-3441.2001

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Heat-Inducible Surface Stress Protein (Hsp70) Mediates Sulfatide Recognition of the Respiratory Pathogen Haemophilus influenzae

Journal article published in 2001 by Evamarie Hartmann, Clifford A. Lingwood, Joachim Reidl ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT The in vitro glycolipid binding specificity of clinical strains of nontypeable Haemophilus influenzae is altered to include sulfated glycolipids following a brief heat shock. We have constructed, expressed, and purified a recombinant protein of H. influenzae Hsp70, which showed significant specific binding to sulfated galactolipids in vitro. Furthermore, indirect immunofluorescence demonstrates that Hsp70 proteins are surface exposed in H. influenzae only after heat shock and are contained in the outer membrane protein fractions.