Published in
American Society for Microbiology, Antimicrobial Agents and Chemotherapy, 1(51), p. 394-396, 2007
DOI: 10.1128/aac.00637-06
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Effects of inoculum size and incubation time on broth microdilution susceptibility testing of lactic acid bacteria
Journal article published in 2006 by
Maria Egerv,
Maria Egervarn,
Hans Lindmark,
Stefan Roos,
Geert Huys 
,
Sven Lindgren
,
Sven Lindgren
This paper is made freely available by the publisher.
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Abstract
ABSTRACT Inoculum size and incubation time were varied during broth microdilution testing of the susceptibilities of 35 strains of lactic acid bacteria to six antibiotics. An increase in either parameter resulted in elevated MICs for all species. An inoculum of 3 × 10 5 CFU/ml is recommended to assess the antibiotic susceptibilities of these bacteria by using broth microdilution.