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Cold Spring Harbor Laboratory Press, Genes & Development, 7(21), p. 750-755, 2007

DOI: 10.1101/gad.1528607

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Two small regulatory RNAs establish opposing fates of a developmental axis

Journal article published in 2007 by Fabio T. S. Nogueira, Shahinez Madi, Daniel H. Chitwood ORCID, Michelle T. Juarez, Marja C. P. Timmermans
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Small RNAs are important regulators of gene expression. In maize, adaxial/abaxial (dorsoventral) leaf polarity is established by an abaxial gradient of microRNA166 (miR166), which spatially restricts the expression domain of class III homeodomain leucine zipper (HD-ZIPIII) transcription factors that specify adaxial/upper fate. Here, we show that leafbladeless1 encodes a key component in the trans-acting small interfering RNA (ta-siRNA) biogenesis pathway that acts on the adaxial side of developing leaves and demarcates the domains of hd-zipIII and miR166 accumulation. Our findings indicate that tasiR-ARF, a ta-siRNA, and miR166 establish opposing domains along the adaxial–abaxial axis, thus revealing a novel mechanism of pattern formation.