Published in

Elsevier, Journal of Molecular Biology, 3(377), p. 774-789, 2008

DOI: 10.1016/j.jmb.2007.12.077

Links

Tools

Export citation

Search in Google Scholar

Genomic and Proteomic Analysis of phiEco32, a Novel Escherichia coli Bacteriophage

Journal article published in 2008 by Dhruti Savalia, Lars F. Westblade, Manisha Goel, Laurence Florens, Priscilla Kemp, Natalja Akulenko, Olga Pavlova, Julio C. Padovan, Brian T. Chait, Michael P. Washburn, Hans-W. Ackermann, Arcady Mushegian ORCID, Tarasii Gabisonia, Ian Molineux, Konstantin Severinov
This paper is available in a repository.
This paper is available in a repository.

Full text: Download

Green circle
Preprint: archiving allowed
Upload
Orange circle
Postprint: archiving restricted
Upload
Red circle
Published version: archiving forbidden
Policy details
Data provided by SHERPA/RoMEO

Abstract

A novel bacteriophage infecting Escherichia coli was isolated during a large-scale screen for bacteriophages that may be used for therapy of mastitis in cattle. The 77,554-bp genome of the bacteriophage, named phiEco32, was sequenced and annotated, and its virions were characterized by electron microscopy and proteomics. Two phiEco32-encoded proteins that interact with host RNA polymerase were identified. One of them is an ECF family sigma factor that may be responsible for transcription of some viral genes. Another RNA polymerase-binding protein is a novel transcription inhibitor whose mechanism of action remains to be defined.