[要旨] 遺伝資源としての生殖細胞の保存および輸送方法の確立のために、従来の超低温下による凍結保存に変わる技術として、新たに私たちは初期胚の低温保存および冷蔵輸送方法を開発した。本研究では、排卵に至らない卵巣内に存在する未成熟卵子を用いて得られた体外受精由来2細胞期胚の冷蔵輸送方法および産子への発生能を検討した。成熟マウス卵巣内よりGV期卵子を回収し体外成熟後に発生したMH期卵子(1,72211,920:90％)を透明帯穿孔処理により体外受精をおこなったところ56％(5521992)の受精成績であり、83％(4571552)が2細胞期胚へと発生した。これらの胚は、近畿大学先端技術総合研究所(和歌山)から熊本大学・CARD(熊本)冷蔵輸送したところ、約48時間後における冷蔵保存胚は、100％(2401240)の生存成績であり、それらの一部の胚を移植した結果、10％(211213)の産子を得た。以上の結果より、体外成熟由来2細胞期胚の冷蔵輸送・冷蔵保存が可能であることが示された。 [Abstract] We developed transport and preservation at cool temperature of early embryo for substitution of traditional vitrification technique to establish preservation and transport method of Bio Resource as for gamete. This study was investigation of transport and preservation at cool temperature and development of live youngs using mice 2-cell stage produced by in vitro matured oocytes. In vitro matured oocytes (90％:1,722/1,920) obtained from C57BL/6J ovary were fertilized using laser optimal system (56％: 552/992). Fertilized eggs were developed to 2-cell stage (83％457/552). Sampling tubes containing 2-cell stage embryos produced by in vitro fertilization were send to the Center for Animal Resources and Development (CARD) , Kumamoto University, Kumamoto from the Institute of Advanced Technology, Kinki University, Wakayama. At CARD, the embryos were recovered from the sampling tubes. The rate of viable 2-cell stage embryos after transport was 100％ (240/240). Collected 2-cell stage embryos were transferred into the oviducts of pseudopregnant females on the day that a vaginal plug was found (Day 1 of pseudopregnancy). Two cell stage embryos transferred to oviducts were developed to live youngs (10％: 21/213).