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American Society for Microbiology, Journal of Clinical Microbiology, 6(52), p. 2139-2143, 2014

DOI: 10.1128/jcm.03389-13

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Inhibition Controls for Qualitative Real-Time PCR Assays: Are They Necessary for All Specimen Matrices?

Journal article published in 2014 by S. P. Buckwalter, L. M. Sloan, S. A. Cunningham ORCID, M. J. Espy, J. R. Uhl, M. F. Jones, E. A. Vetter, J. Mandrekar, F. R. Cockerill, B. S. Pritt ORCID, R. Patel, N. L. Wengenack
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT A retrospective analysis of 386,706 specimens representing a variety of matrix types used in qualitative real-time PCR assays determined the overall inhibition rate to be 0.87% when the inhibition control was added preextraction to 5,613 specimens and 0.01% when the inhibition control was added postextraction but preamplification in 381,093 specimens. Inhibition rates of ≤1% were found for all specimen matrix types except urine and formalin-fixed, paraffin-embedded tissue.