Este artículo contiene 14 páginas, 3 tablas, 3 figuras. ; Aim: We studied global variation in beta diversity patterns of lake macrophytes using regional data from across the world. Specifically, we examined (1) how beta diversity of aquatic macrophytes is partitioned between species turnover and nestedness within each study region, and (2) which environmental characteristics structure variation in these beta diversity components. Location: Global. Methods: We used presence–absence data for aquatic macrophytes from 21 regions distributed around the world. We calculated pairwise-site and multiple-site beta diversity among lakes within each region using Sørensen dissimilarity index and partitioned it into turnover and nestedness coefficients. Beta regression was used to correlate the diversity coefficients with regional environmental characteristics. Results Aquatic macrophytes showed different levels of beta diversity within each of the 21 study regions, with species turnover typically accounting for the majority of beta diversity, especially in high-diversity regions. However, nestedness contributed 30–50% of total variation in macrophyte beta diversity in low-diversity regions. The most important environmental factor explaining the three beta diversity coefficients (total, species turnover and nestedness) was elevation range, followed by relative areal extent of freshwater, latitude and water alkalinity range. Main conclusions: Our findings show that global patterns in beta diversity of lake macrophytes are caused by species turnover rather than by nestedness. These patterns in beta diversity were driven by natural environmental heterogeneity, notably variability in elevation range (also related to temperature variation) among regions. In addition, a greater range in alkalinity within a region, likely amplified by human activities, was also correlated with increased macrophyte beta diversity. These findings suggest that efforts to conserve aquatic macrophyte diversity should primarily focus on regions with large numbers of lakes that exhibit broad environmental gradients. ; The gathering of the Finnish data was partly supported by Biological Monitoring of Finnish Freshwaters under diffuse loading -project (XPR3304) financed by Ministry of Agriculture and Forestry and partly by national surveillance monitoring programs of lakes. S.H. and M.M. were supported by the EU-funded MARS-project (7th EU Framework Programme, Contract No.: 603378).Swedish macrophyte data were collected within the Swedish Monitoring Program of macrophytes in lakes funded by the Swedish Agency for Marine and Water Management. S.K. was supported by NWO Veni grant 86312012. Macrophyte data from Brazilian Amazon were collected within a limnological monitoring program funded by Vale S.A. The vast majority of macrophyte data from Polish lakes were collected within the State Environmental Monitoring Programme and were provided by the Inspection for Environmental Protection. Macrophyte data for British lakes were collated by the Joint Nature Conservation Committee from surveys resourced by the national conservation agencies. Swiss macrophytes data were collected during a study financially supported by the Swiss Federal Office for the Environment. Wisconsin data collection was funded by the Wisconsin Department of Natural Resources and supported by the Wisconsin Cooperative Fishery Research Unit. The Norwegian macrophyte data were collected within the European Union project ‘LAKES – Long distance dispersal of Aquatic Key Species’, contract no. env4-ct-97-0585. ; Peer reviewed