Chronic inflammatory diseases may develop when reguZatory Tcells (Tregs)fail to control the balance between tolerance and immunity. Alternatively, activated immune cells might prevent the induction or activation of Tregs in such diseases. In this study, we demonstrate that trans-signaling into T cells via the soluble IL-6 receptor completely abrogates the de novo induction of adaptive Tregs. Mechanistically, I -6trans-signaling augmented the expression of the TGF-beta signaling inhibitor SJVL4D7.- Consequently, SMAD7 overexpression in T cells using newly created transgenic mice rendered CD4(+) CD25(-) T cells resistant to the induction of ToxP3. Finally, IL-6 trans-signaling inhibited Treg-mediated suppression in a murine model Of colitis. In summary, IL-6 trans-signaling into T cells emerges as a key pathway for blockade of the development of adaptive Tregs and thus may play a pivotal role in shifting the balance between effector and regulatory T cell numbers in chronic inflammatory and autoimmune diseases.