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EMBO Press, EMBO Reports, 12(3), p. 1215-1221, 2002

DOI: 10.1093/embo-reports/kvf235

EMBO Press, EMBO Reports, 5(4), p. 534-534

DOI: 10.1038/sj.embor.embor850

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COP9 signalosome components play a role in the mating pheromone response of S. cerevisiae

Journal article published in 2002 by Ron Piran, Elah Pick, Kay Hofmann, Vered Maytal-Kivity, Michael H. Glickman
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

A family of genetically and structurally homologous complexes, the proteasome lid, Cop9 signalosome (CSN) and eukaryotic translation initiation factor 3, mediate different regulatory pathways. The CSN functions in numerous eukaryotes as a regulator of development and signaling, yet until now no evidence for a complex has been found in Saccharomyces cerevisiae. We identified a group of proteins, including a homolog of Csn5/Jab1 and four uncharacterized PCI components, that interact in a manner suggesting they form a complex analogous to the CSN in S. cerevisiae. These newly identified subunits play a role in adaptation to pheromone signaling. Deletants for individual subunits enhance pheromone response and increase mating efficiency. Overexpression of individual subunits or a human homolog mitigates sst2-induced pheromone sensitivity. Csi1, a novel CSN interactor, exhibits opposite phenotypes. Deletants also accumulate Cdc53/cullin in a Rub1-modified form; however, this role of the CSN appears to be distinct from that in the mating pathway.