Other ; The phylogenetic information inferred from the study of homologous genes helps us to understand the evolution of gene families and plays a vital role in finding ancestral gene duplication events as well as identifying regions that are under positive selection within species. The Ensembl GeneTrees pipeline generates gene trees based on coding sequences and provides details about exon conservation, and is used in the Ensembl Compara project to discover homologous gene families. Since expertise is required to configure and run the pipeline via the command-line, we created GeneSeqToFamily, an open-source Galaxy workflow based on Ensembl GeneTrees. GeneSeqToFamily helps users to run potentially large-scale gene family analyses without requiring the command-line while still allowing tool parameters, configurations, and the tools themselves to be modified.