Published in

Nature Research, Nature Structural and Molecular Biology, 1(18), p. 88-90, 2010

DOI: 10.1038/nsmb.1952

Links

Tools

Export citation

Search in Google Scholar

Cryo-EM structure of the E. coli translating ribosome in complex with SRP and its receptor

Journal article published in 2010 by Leandro F. Estrozi ORCID, Daniel Boehringer, Shu-Ou Shan, Nenad Ban, Christiane Schaffitzel
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

Full text: Download

Green circle
Preprint: archiving allowed
Upload
Orange circle
Postprint: archiving restricted
Upload
Red circle
Published version: archiving forbidden
Policy details
Data provided by SHERPA/RoMEO

Abstract

We report the 'early' conformation of the Escherichia coli signal recognition particle (SRP) and its receptor FtsY bound to the translating ribosome, as determined by cryo-EM. FtsY binds to the tetraloop of the SRP RNA, whereas the NG domains of the SRP protein and FtsY interact weakly in this conformation. Our results suggest that optimal positioning of the SRP RNA tetraloop and the Ffh NG domain leads to FtsY recruitment.