The geochemical analyses of karst springs and their freshwater carbonate deposits provide an opportunity to reconstruct past climate changes. Nevertheless, there are still very few paleoclimate records obtained from freshwater carbonate deposits in Hungary. The present study focuses on some recently depositing freshwater tufa sites and two caves located in Mecsek Mts (Southern Hungary) as possible sources for Holocene paleoclimate research. Both carbonate and water samples were collected for stable isotope analyses in June and August 2011 and a monitoring programme was started in October 2011 at five sites. The stable isotope analyses of the rock samples reflect the effect of continentality and suggest strong soil zone CO2 contribution.