Background: Neck injuries are the most frequent traumatism in rugby at all levels of the game. Strengthening and mobilizing the neck musculature has often been indicated as a possible strategy to reduce the perceived level of muscle soreness and number of neck injuries. Still, the efficacy of such intervention is under debate. Purpose: This study assessed the efficacy of a 12-week training for neck muscle strength and mobility in reducing the level of perceived soreness in amateur rugby players. Methods: The perceived soreness of the neck muscles was recorded before, immediately and 24 h after 12 rugby matches in amateur rugby players (NT, n = 12) and controls (Ctrl, n = 11). The neck range of motion (ROM), the maximum voluntary force (MVC), and surface electromyogram (EMG) of the neck muscles were evaluated before and after 12-week neck muscle training (3 sessions per week, 35 min each). Training consisted of isometric and concentric contractions, and passive stretching of the neck muscles. From EMG, the root mean square (RMS) was calculated. Results: After 4 weeks, the perceived soreness of neck muscles was lower in NT than in Ctrl (P