Dissemin is shutting down on January 1st, 2025

Published in

Nature Research, Nature Nanotechnology, 2(12), p. 177-183, 2016

DOI: 10.1038/nnano.2016.228

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Nanomechanical mapping of first binding steps of a virus to animal cells.

Journal article published in 2016 by David Alsteens ORCID, Richard Newton, Rajib Schubert ORCID, David Martinez-Martin ORCID, Martin Delguste, Botond Roska, Daniel J. Müller ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Viral infection is initiated when a virus binds to cell surface receptors. Because the cell membrane is dynamic and heterogeneous, imaging living cells and simultaneously quantifying the first viral binding events is difficult. Here, we show an atomic force and confocal microscopy set-up that allows the surface receptor landscape of cells to be imaged and the virus binding events within the first millisecond of contact with the cell to be mapped at high resolution (