Research team head ; Research team member ; Research team member ; Publisher ; 研究種目 : 挑戦的萌芽研究 研究期間 : 2014～2015 課題番号 : 26670433 研究分野 : 腎臓病学, 内分泌学, 代謝学 ; グレリン(Ghr)は腎障害をミトコンドリア(Mit)由来酸化ストレス低下を介して治癒する。一方腎障害におけるMitダイナミックスの意義が注目されている。糖尿病の腎障害において, Ghrの有効性を検証した。8週齢のC57BL/6マウスへストレプトゾトシンを投与し糖尿病発症を確認した後, 12週間 Ghr(300μg/kg)を連日腹腔投与した。その結果Ghrが糖尿病におけるMitの膨化, クリステの断裂を軽快させた。GhrはMitのfissionおよびfusionに影響を与えなかったが, マイトファジー関連分子であるPINK1およびParkinの発現を上昇させた。グレリンが糖尿病性腎症の悪化を抑制する。 Ghrelin have protective effects against renal impairment by reducing renal mitochondria-derived oxidative stress and by improving mitochondrial function. Recent study revealed that mitochondria dynamics plays an important role in the initiation and progression of renal failure. We investigate the efficacy of Ghrelin for the treatment of diabetic kidney impairment. C57BL/6 mice at the age of 8 weeks were subjected to streptozotosin injection in order to render diabetic. After the onset of diabetes, Ghrelin at the dose of 300μg/kg was administered by daily intraperitoneal injection. We found that swelling of mitochondria and the disruption of mitochondrial cristae in the kidney were ameliorated by the treatment with Ghrelin. Ghrelin increased the expressions of genes related to mitophagy including PINK1 and Perkin, although it did not have any effect on the genes related to mitochondria fission or fusion. We concluded that Ghrelin has renal protective effects in diabetic kidney disease.