Anthocyanins are water soluble pigments used as natural colorants which have been extendedly studied due to their beautiful colors, and more important, beneficial health potential. Published studies regarding anthocyanins health benefits have been performed using crude anthocyanin extracts from natural sources with no any data about the purity of the tested extract. The impurities from the crude extracts are mostly other phenolic compounds; which can also have biological proprieties. In this case the beneficial potential of crude extract is not attributed only to anthocyanin. Thus, data regarding anthocyanins purity are required for the biological research. Currently, most investigations on anthocyanins are focused on their stability, as well as their purification and identification. In this paper, the most recent techniques in the chemical investigation of the anthocyanins purification are summarized.