Additional file 2: Figure S2. of Generation of monoclonal antibodies against native viral proteins using antigen-expressing mammalian cells for mouse immunization
Published in 2016 by
Natalie Rose,
Carlos Pinho-Nascimento,
Alessia Ruggieri,
Paola Favuzza,
Marco Tamborrini,
Hanna Roth,
Marcia Baroni de Moraes,
Hugues Matile,
Thomas Jänisch,
Gerd Pluschke,
Katharina Röltgen
Abstract
Expression of D2NS1 and D3NS1 by transfected HEK cells. While aqua staining of lysates prepared from HEK-derived cell lines expressing D2NS1 (D2) and D3NS1 (D3) showed no specific band for the NS1 protein as compared to the lysates of untransfected HEK cells (−) (a), Western blot analysis using anti-hexa-His tag antibodies confirmed the expression of D2NS1 and D3NS1 by the HEK cells (b). Western blotting using anti-tubulin antibodies was performed as a control for the amount of untransfected and transfected HEK cell lysates loaded on the gels (c). M = molecular weight marker in kDa. (TIF 3371 kb)