Expression of D2NS1 and D3NS1 by transfected HEK cells. While aqua staining of lysates prepared from HEK-derived cell lines expressing D2NS1 (D2) and D3NS1 (D3) showed no specific band for the NS1 protein as compared to the lysates of untransfected HEK cells (−) (a), Western blot analysis using anti-hexa-His tag antibodies confirmed the expression of D2NS1 and D3NS1 by the HEK cells (b). Western blotting using anti-tubulin antibodies was performed as a control for the amount of untransfected and transfected HEK cell lysates loaded on the gels (c). M = molecular weight marker in kDa. (TIF 3371 kb)