Additional file 4: Figure S1. Extended analyses of SVA-D. (A) BORIS binding peaks at SVA VNTRs in Delta-47 cells. The ChIP-seq tag density distribution for the full-length SVA-D element from Repbase indicates that BORIS retains preference for VNTR region event in this cell line completely unrelated to K562 and with a substantially lower BORIS expression level. The normalized counts were binned along the DNA sequence (histogram bars) with the smoothing line added. (B) The assessment if CpG methylation of oligonucleotides used in EMSA. DNA fragments were digested by the methylation-sensitive endonuclease Aci I before and after methylation.