CTCF&BORIS bound regions enclose at least two CTCF binding motifs. a The motifs identified by MEME for CTCF-only, CTCF&BORIS and BORIS-only bound regions in K562 cells. Additionally, the presence of the CTCF motif was confirmed in 70 % of BORIS-only bound regions. b, c The alignment of human and mouse sequences representing five CTCF&BORIS bound regions (b) and two CTCF-only bound regions (c). The summits of CTCF and BORIS peaks are highlighted by red and blue nucleotides, respectively. The CTCF motif is shown in bold italics and underlined. The CTCF motif is indicated with black and blue colors, depending on sense and antisense orientation, respectively. d, e Number of CTCF motifs in the genomic regions, encircling 100 bp upstream and downstream of the summit of CTCF peaks at CTCF&BORIS bound regions (d) and from the summit of CTCF-only peaks (e). The histogram shows the percentage of CTCF binding regions (y-axis) containing 0 to 7 CTCF motifs (x-axis). f The distribution of distances between two CTCF motifs at 1-bp resolution (x-axis) at CTCF&BORIS bound regions (y-axis). The CTCF&BORIS bound regions that have only two CTCF motifs under the peaks were selected for the analysis. (PPTX 3766 kb)